Avaliação da técnica de inoculação manual de solução salina na câmara de ar e no albúmen e sua influência sobre a eclodibilidade e desenvilvimento embrionário

This work is the result of an experimental study conducted at the Federal Institute of Education, Science and Technology of Minas Gerais – Campus Bambuí, aimed at evaluating the effects of the manual inoculation technique with saline solution (physiological saline) in the albumen and air cell of fertile eggs, performed on day 0 of incubation, on hatchability and embryonic development. One hundred and fifty fertile eggs from free-range hens at 40 weeks of age were used, divided into three treatments: CP - positive control (no inoculation), ALB - inoculation in the albumen, and CA - inoculation in the air cell, with each group consisting of 50 eggs. Statistical analysis was conducted using the Kruskal-Wallis test. The results indicated that inoculation in the albumen led to a higher discard rate and increased early embryonic mortality, numerically reducing the number of hatched chicks, although statistically, the treatments did not show significant differences (P>0.05). On the other hand, inoculation in the air cell showed performance similar to the control group, suggesting it as a viable alternative. It was concluded that the inoculation of 0.2 mL of saline solution in the albumen on the first day of incubation is not recommended, as it may compromise the internal egg structure and negatively interfere with embryonic development. The technique applied in the air cell shows promise and may be considered in future research aimed at early in ovo nutrition. The study highlights the need for further investigation to determine the ideal location and volume of solution inoculation in incubatable eggs.